Directed evolution: an evolving and enabling synthetic biology tool
نویسندگان
چکیده
منابع مشابه
Directed evolution: an evolving and enabling synthetic biology tool.
Synthetic biology, with its goal of designing biological entities for wide-ranging purposes, remains a field of intensive research interest. However, the vast complexity of biological systems has heretofore rendered rational design prohibitively difficult. As a result, directed evolution remains a valuable tool for synthetic biology, enabling the identification of desired functionalities from l...
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At the heart of synthetic biology lies the goal of rationally engineering a complete biological system to achieve a specific objective, such as bioremediation and synthesis of a valuable drug, chemical, or biofuel molecule. However, the inherent complexity of natural biological systems has heretofore precluded generalized application of this approach. Directed evolution, a process which mimics ...
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Directed evolution is a powerful algorithm for engineering proteins to have novel and useful properties. However, we do not yet fully understand the characteristics of an evolvable system. In this chapter, we present examples where directed evolution has been used to enhance the performance of metalloenzymes, focusing first on ‘classical’ cases such as improving enzyme stability or expanding th...
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ژورنال
عنوان ژورنال: Current Opinion in Chemical Biology
سال: 2012
ISSN: 1367-5931
DOI: 10.1016/j.cbpa.2012.05.186